Vimentin positivity has a limited value in the diagnosis of soft tissue tumors; however, if the mesenchymal tissue is negative for vimentin, it may indicate that the tissue is suboptimal for IHC or not of a mesenchymal (soft tissue) differentiation Myofibroblastic tumor of soft tissue displaying desmin-positive and actin-negative immunophenotypes Myofibroblasts have ultrastructural and functional characteristics intermediate between fibroblasts and smooth muscle cells. Previous studies indicated that most myofibroblasts express actin and vimentin but not desmin immunophenotypes
carcinoma, melanoma and follicular dendritic cell sarcoma can present in soft tissue locations and thereby enter the differential diagnosis. Synovial sarcoma is diagnostically obvious when biphasic. The monophasic form is a spindle cell sarcoma composed of closely packed uniform cells with overlappin Immunohistochemistry plays a key role in the diagnosis of soft tissue tumors. Until recently, however, the primary purpose of immunohistochemistry in this context was simply to attempt to. Tumor cells were oval to spindle-shaped myoid cells with bland appearance. Immunohistochemically, vimentin, calponin and CD34 were positive, while α-smooth muscle actin, h-caldesmon, HHF35 and desmin were negative. Although CD34 was positive, the present case could be diagnosed as infantile myofibromatosis Vimentin staining in a lymphoma. Vimentin is a common immunostain. It marks most soft tissue lesions and malignant melanoma. It is usually negative in carcinomas Normal testicular tissue and 76 testicular germ-cell tumors of various types were immunohistochemically evaluated for the expression of intermediate filament proteins of different types. In normal testes, the rete testis epithelium was positive to cytokeratin, and the Sertoli cells, stromal cells, and Leydig cells were positive for vimentin
Update on soft tissue tumors. A, Low-grade spindle cell sarcoma with immature adipocytes and non-pleomorphic lipoblasts in the low-grade dedifferentiated component (case 1). B, Within the same tumour, high-grade spindle cell sarcoma containing lipoblasts with variable nuclear pleomorphism (case 1). C, Lipogenic low-grade myxoid liposarcoma- Certain types of soft tissue tumor are positive for CD99. CD99 is a membranous protein that is expressed in most cases of Ewing sarcoma (EWS), synovial sarcoma (SS) and low-grade fibromyxoid sarcoma (LGFMS), although its involvement in these diseases is unknown . The neoplasms were within the superficial subcutaneous soft tissue with minimal or no involvement of deep musculature. Most tumors behaved in an indolent fashion Thirteen primary and metastatic testicular germ cell tumours, including classical and anaplastic seminomas, and non-seminomatous testicular tumours were examined for their intermediate filament protein (IFP) types. The seminomas were shown to react with a monoclonal and a polyclonal antibody to bovine lens vimentin, while non-seminomatous germ cell tumours were strongly positive for a. ought to validate these results in a larger series of soft tissue neoplasms of both neural crest and non-neural crest origin, and to further characterize the sensitivity and specificity of Sox10 for use in clinical diagnosis. We evaluated Sox10 and S100 mRNA levels in 122 cases of peripheral nerve sheath tumors and synovial sarcoma and used immunohistochemistry for Sox10 and S100 protein.
Immunohistochemically, the tumor cells were diffusely positive for Vimentin, and partially positive for epithelial membrane antigen (EMA) with at least moderate intensity, which was mainly expressed in epithelioid cells (Fig.2a). Estrogen receptor (ER) was weakly expressed in some tumor cells (Fig.2b). Calponin (clone: CALP and EP63) was positive for both the nucleus and the cytoplasm of the. Seven feline soft tissue tumors histologically diagnosed as rhabdomyosarcomas were typed immunohistochemically for desmin, vimentin, glial fibrillary acidic protein, and neuro- filament proteins. All cats were European short hairs. The tumors were at different locations, i.e., at the larynx, mam • In some mesenchymal tissues vimentin is typically co-expressed along with the type-specific intermediate filaments e.g. Desmin and Vimentin co- expression in muscle cells Vimentin and GFAP in some Schwann cells • Absence may be clue to rare tumors like alveolar soft part sarcoma and perivascular epitheloid cell neoplasm Myxoid soft-tissue sarcomas represent a heterogeneous group of mesenchymal tumors characterized by a predominantly myxoid matrix, including myxoid liposarcoma (MLS), low-grade fibromyxoid sarcoma (LGFMS), extraskeletal myxoid chondrosarcoma (EMC), myxofibrosarcoma, myxoinflammatory fibroblastic sarcoma (MIFS), and myxoid dermatofibrosarcoma protuberans (DFSP)
Subcutaneous proliferation of bland fibrous tissue which radiates outward from center of lesion. Tumors have nodules of histiocyte-like cells. and giant cells, fibrous tissue and hemorrhage. Histiocyte-like cells, pale staining cytoplasm; giant cells present. Nodules may be composed of cells w/ elongated nuclei Background and Aims: Soft tissue tumors are relatively rare in general pathology, but in many cases the diagnosis is difficult because of the complex histogenesis and similar microscopic aspects Tumors that metastasize to lymph nodes. Rhabdomyosarcoma. A soft tissue tumor that can be easily identified with its cytology. Vimentin. Stains and differentiates all sarcoma with MESENCHYMAL origin. Differentiates other Epithelial tumors: Renal Cell Carcinoma, Endometrial Endometroid Type of Adenocarcinoma
Immunostains are positive for desmin, myosin and vimentin. Treatment is usually wide surgical excision with chemotherapy or radiation depending on the sub-type. Epidemiology. Most common soft tissue sarcoma in the foot. 4% (80/1934) 2. Most common soft tissue sarcoma in a child. 79% (1530/1934) 3. Commonly shows calcification on plain. . The presence of desmin was confined to leiomyosarcomas and rhabdomyosarcomas, which usually showed desminpositivity heterogeneous tumors immunohistochemically, but the ma-jority stain strongly for vimentin and variably for S100 pro-tein and NSE.9,13,15 In these cases, both cutaneous tumors were strongly positive for vimentin and fewer cells were positive for NSE and laminin. None of the tumors was pos-itive for S100 protein. Both cutaneous tumors were strongl Abstract Mixed tumor of soft tissue is extremely rare; only 15 cases have been reported in English literature. A 56-year-old man presented with a tumor in right arm. positive for vimentin.
Louis Guillou, Andrew L. Folpe, in Bone and Soft Tissue Pathology, 2010. D ifferential Diagnosis. The differential diagnosis of soft tissue giant cell tumor includes both benign and malignant entities. Essentially any malignant neoplasm may contain large numbers of osteoclast-like giant cells on occasion, including various sarcomas, melanoma, carcinomas, and even lymphomas . As with the first case, positive staining for vimentin was indicative of a tumor of mesenchy-mal origin. Figure 1. Laterolateral contrast arthrogram of the left metacar-pophalangeal (MCP) joint from case 1. A smooth soft tissue density mass (approximately 40 mm × 40 mm) can be seen outlined a
Scattered tumor cells were positive for MDM2 immunostain, but MDM2 amplification was not detected using fluorescent in situ hybridization (FISH). Co-expression of cytokeratin and vimentin by the tumor raised the possibility of metastatic renal cell carcinoma and positivity of the tumor for PAX8 supported this hypothesis soft tissue locations, histologic grade and clinical stage are the most useful parameters for assessing prognosis. Cancer 3994; 74:2251-60. Key words: mediastinum, leiomyosarcoma, smooth mus- cle tumors, immunohistochemistry, electron microscopy, epithelioid leiomyosarcoma, soft tissue tumors. Malignant smooth muscle neoplasms presenting as me Epithelioid cell features mimicking carcinomas characterize a variety of histogenetically, phenotypically, and molecularly distinct subsets of mesenchymal neoplasms. In a pathogenetic sense, epithelioid soft tissue tumors basically fall into three main genetic categories: (1) switch/sucrose non-fermenting (SWI/SNF) complex-deficient tumors (with epithelioid sarcoma as their prototype); (2. Tumors of the Soft Tissues, Atlas of Tumor Pathology, AFIP Third Series, Fascicle 30, 2001; Fletcher CDM, Unni KK, Mertens F. Pathology and Genetics of Tumours of Soft Tissue and Bone, World Health Organization Classification of Tumours 2002; Weiss SW, Goldblum JR. Enzinger and Weiss's Soft Tissue Tumors, 4th edition, 200
Despite their similar IHC staining profiles, clear cell sarcoma of soft tissue is clinically and genetically distinct from cutaneous melanoma.155 Like the latter, it is positive for melanocytic markers S100, HMB-45, and less consistently for melan (Mart-1).156-158 Other positive markers include neuron-specific enolase, CD57, and vimentin Cytokeratin, vimentin, and S100 protein are positive. Variable results are seen with immunohistochemistry and are rarely of diagnostic value. It reacts with EMA, S-100 protein, and Bcl-2; these findings can help differentiate it from PA and ACC. Synovial sarcoma is a malignant soft tissue tumor that shows epithelial and mesenchymal. CD99 (MIC2) represents the monoclonal antibody to EWSR1-FLI1 fusion product and is expressed in a membranous pattern. FLI1 and NKX2.2 yield a strong nuclear stain, while vimentin is expressed in nearly all ES/PNET tumors. NSE and synaptophysin are expressed less often. Uncommonly, S100 protein, GFAP, and keratin will also be expressed It is a benign tumor but have a tendency to recur, and bleed again, and again, and again. It is a tumor of young-aged patients, mostly adolescents younger than 20 years. The tumor affects mostly males, a fact related to its immunoreactivity for androgen receptors (M>F). It is not a bugger, it's a juvenile nasopharyngeal angiofibroma
Foci resembling solitary fibrous tumor, fibromatosis, keloid or inflammatory myofibroblastic tumor were observed. Immunohistochemical study showed that the tumor cells were diffusely positive for vimentin and focally positive for CD34, factor VIII-related antigen and beta-catenin 1. Pitfalls in diagnosis of soft tissue tumors of childhood Presenter: Dr. Sonic V.S Moderator: Dr. Sumita Tripathy Dept Of Pathology, MKCG Medical College, Berhampur, Odisha. 2. • Soft tissue is defined as nonepithelial extraskeletal tissue of the body exclusive of the reticuloendothelial system, glia, and supporting tissue of parenchymal.
Tumors of the Soft Tissues, Atlas of Tumor Pathology, AFIP Third Series, Fascicle 30, 2001; Fletcher CDM, Unni KK, Mertens F. Pathology and Genetics of Tumours of Soft Tissue and Bone, World Health Organization Classification of Tumours 2002; Weiss SW, Goldblum JR. Enzinger and Weiss's Soft Tissue Tumors, 4th edition, 2001; Folpe AL, Weiss SW Extrathoracic solitary fibrous tumors have been described at almost every anatomic location [1-3], but reports of tumors at the extremities or intramuscular tumors as well as those with malignant clinical behavior or atypical histologic features are rare .Few cases of soft tissue epithelioid solitary fibrous tumor affecting mediastinum , orbit , neck  and ischioanal fossa [7, 8] have. Recent advances in soft tissue tumors. round cell tumor etc. Bcl-2 • It is a family of protein involved in the apoptosis pathway in cell grown and death • Positive in Solitary fibrous tumor, Synovial sarcoma, DFSP • CD31 has been the gold standard as a marker for endothelial differentiation • Diagnosis of malignant vascular tumours. subcutaneous and soft tissue canine tumors were examined for immunoreactivity to cytokeratin, desmin, and vimentin. The avidin-biotin complex (ABC) technique demonstrated immunoreactivity in both.
Synovial Sarcoma. Synovial Sarcoma is a malignant, soft tissue sarcoma caused by a t (X;18) chromosomal translocation mutation most commonly found near joints, but rarely within the joint. The condition usually presents in patients between 15 and 40 years old with a growing mass in proximity to a joint Keratin positive soft tissue tumor composed of large epithelioid cells almost always involving distal extremities (see also proximal type epithelioid sarcoma) Alternate/Historical Names. Classical type epithelioid sarcoma ; Large cell epithelioid sarcoma; Diagnostic Criteria . 95% keratin positive. CD34 50 2. • Soft tissue is defined as complex of nonepithelial extra skeletal structure of body exclusive of supportive tissue of various organs and the hematopoietic/ lymphoid tissue. 3. • It is composed of fibrous tissue, adipose tissue, skeletal muscle, blood and lymph vessels and peripheral nerve. • Most of the soft tissue derived from. Desmin and Actin are positive (Fig. 9,10 - above) Vimentin Positive Keratin negative PROGNOSIS Biological Behavior Tumors located in an extremity have 50% survival at 5 years. Retroperitoneal tumors have 25-35% 5 year survival Earlier diagnosis with deep soft tissue masses and subcutaneous tumors compared to retroperitoneal tumors More easily. 3 histologic patterns for soft tissue tumors. 1. spindle cell: nuclei rod-shaped; 2x as long as wide. 2. small round blue cell. 3. epithelioid. basic immunostains that aid in Dx. -S-100: nerve related; melanoma. -Vimentin: sarcomas (some carcinomas
Enzinger and Weiss's Soft Tissue Tumors, 4th edition, 2001 Hill DA, Pfeifer JD, Marley EF, Dehner LP, Humphrey PA, Zhu X, Swanson PE. WT1 staining reliably differentiates desmoplastic small round cell tumor from Ewing sarcoma/primitive neuroectodermal tumor Desmoid tumors are also known as aggressive fibromatosis or desmoid-type fibromatosis. A desmoid tumor can occur anywhere in the body since connective tissue is found everywhere in your body. Desmoid tumors are often found in the abdomen, as well as the shoulders, upper arms, and thighs. Desmoid tumors are benign, which means they are not cancer
Introduction. Certain types of soft tissue tumor are positive for CD99. CD99 is a membranous protein that is expressed in most cases of Ewing sarcoma (EWS), synovial sarcoma (SS) and low-grade fibromyxoid sarcoma (LGFMS), although its involvement in these diseases is unknown Mammary-type myofibroblastoma of soft tissue: a tumor closely related to spindle cell lipoma. Am J Surg Pathol. 2001 Aug;25(8):1022-9. Pauwels P, Sciot R, Croiset F, Rutten H, Van den Berghe H, Dal Cin P. Myofibroblastoma of the breast: genetic link with spindle cell lipoma Extrarenal rhabdoid tumors of soft tissue: A clinicopathologic and immunohistochemical study of 18 cases Julie Fanburg-Smith , Mitra Hengge, Ulrich R. Hengge, Joseph S.C. Smith, Markku Miettinen Department of Pediatric
Core needle biopsy of calcaneum was suggestive of EHE. Limb salvage was difficult as the tumor was recurrent and involved the skin and the weight-bearing part of the calcaneum. The patient was managed with trans-tibial amputation. Immunohistochemical (IHC) staining of the excised tissue was positive for CD34, Vimentin, SMA, and Fli-1
Tumor protein 63 (p63) is a transcription factor of the p53 gene family involved in differentiation of several tissues including squamous epithelium. p63 immunohistochemistry is broadly used for tumor classification but published data on its expression in cancer is conflicting. To comprehensively catalogue p63 expression, tissue microarrays (TMAs) containing 12,620 tissue samples from 115. Incidence and Risk Factors. Soft tissue sarcomas are a heterogeneous population of mesenchymal tumors that comprise 15% and 7% of all skin and subcutaneous tumors in the dog and cat, respectively. 1 The annual incidence of soft tissue sarcomas in companion animals is about 35 per 100,000 dogs at risk and 17 per 100,000 cats at risk. 2 In dogs, sarcomas have been associated with radiation. to be a soft tissue sarcoma and classified in the World Health Organization classification of tumors under fibrous positive and negative control tissues was done using staining pattern of vimentin and CD11c, GCTSPs in horses are likely to be giant cell-rich type sarcoma o A ll the tumors were positive . for Vimentin, Soft tissue sarcoma is one of the key areas in surgical pathology where immunohistochemistry plays an important role in both precise diagnosis and. Vimentin, a type III intermediate filament, is highly expressed in aggressive epithelial cancers and is associated with increased rates of metastasis. We show that vimentin is causally required for lung cancer metastasis using a genetic mouse model of lung adenocarcinoma ( LSL - Kras G12D; Tp53 fl/fl, termed KPV +/+) crossed with vimentin-null mice (thereby creating KPV −/− mice)
Synovial sarcoma is a soft tissue tumor occurring in the biphasic or monophasic type and consisting of epithelial and/or spindle cell components. The differential diagnosis includes malignant peripheral nerve sheath tumors, muscle-derived sarcomas, and biphasic mesotheliomas. PAS - positive in pseudoglandular spaces Vimentin - positive CD99. TUMOR TISSUE REGISTRY Soft Tissue Tumors Study Cases, Subscription A April, 2007 California Tumor Tissue Registry c/o: Department of Pathology and Human Anatomy LomaLinda University Sebool of Medicine 11021 Campus f\yenue, AH335 Lorna Linda, California 92350 (909) 558-4788 FAX: (909) 558-0188 E-mail: Cfirstname.lastname@example.org
Of seven benign soft tissue tumors, one ganglioneuroma and one lipomatosis showed low levels of MDR1 expression. By RNA dot-blot analysis, MDR1 transcripts were detectable in 12 of 34 specimens (35%) Cytology of Neoplasia: An Essential Component of Diagnosis. Anne Barger, DVM, MS, Diplomate ACVP. Cytology is a quick, easy, and inexpensive diagnostic tool. It is commonly used for the diagnosis of neoplastic processes. Cytology has several benefits: The risks of fine-needle aspiration are minimal. Anesthesia or sedation is often not required Soft Tissue Tumors, Joint Pathology. STUDY. PLAY. Lipoma. M/C benign soft tissue tumor. Mature fat cells that are well encapsulated. Mobile, painless, slow growing. Liposarcoma. Malignant tumor or fat cells. M/C sites are retroperitoneum, deep soft tissue of proximal extremities
Although the tumors showed distinct morphological and immunohistochemical features enabling delimitation from other soft tissue lesions, they may overlap histologically with myoflbrobiastoma of the breast, lymph node and soft tissue, low grade malignant nerve sheath tumor, lelomyosarcoma, cellular schwannoma, inflammatory flbrosarcoma and. . Although many of these tumors are identified with imaging performed for unrelated reasons, most present with nonspecific symptoms that can lead to a delay in diagnosis. Immunohistochemically, these tumors are positive for vimentin, actin. Likewise, Vimentin expression increased in accordance with tumor progression ( Figure 3B), and the tumors with high expression of Vimentin had a greater extent of tumor invasion to the serosa ( P = 0.003), lymph node metastasis ( P = 0.016) and liver metastasis ( P = 0.0063) than those with low expression of Vimentin ( Table I) Immunohistochemical staining was positive for CD68, smooth muscle actin, and vimentin, but was negative for a panel of epithelial and additional muscle markers. These features were most consistent with GCT-ST, an uncommon neoplasm of low malignant potential. abstract = Primary giant cell tumor of soft tissue (GCT-ST) arising in breast is.
Infiltration and Mitotic Activity in Canine Soft Tissue Sarcomas Iba-1 positive, non-neoplastic, tumor inﬁltrating cells . The whole TAM population tissues were available for review and if neoplastic cells stained positive for vimentin, and negative for the calcium-binding adapter molecule 1 (Iba-1), Von Willebrand factor,. The findings that tumor cells were partially positive for both cytokeratin and vimentin and that both the sacral and hand lesions showed similar pathological features and are positive for prostate specific acid phosphatase and androgen receptor strongly suggested that the tumor was metastatic rather than a primary soft tissue sarcoma Introduction . Soft tissue sarcomas (STSs) represent 1 percent of all adult malignancies and sarcomas only rarely spread to the regional lymph nodes. Case Presentation . We present a case of a woman with a dermatofibrosarcoma protuberans and a sarcoma not therwise specified of the lower extremity. The patient had no distant metastasis during follow-up, but did develop a regional lymph. The word sarcoma represents over fifty subtypes of soft tissue malignant tumors of mesenchymal origin. In 2013, the World Health Organization (WHO) Classification of Tumors of Soft Tissue and Bone has been published, according to the pathogenesis of soft tissue and bone tumors related to the histologic and genetic findings [3, 4] Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all animal cells as well as bacteria. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton.All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal.
An uncommon deep soft tissue tumor Seen in children and teenagers Associated with Kasabach Meritt syndrome especially if retroperitoneal mass > 20 cm M/E : - Lobular architecture, Highly cellular In Individual lobules there is capillary sized vessels with absence of mitosis or cytologic atypia - Microscopic features overlap with hemangioma 37 We describe 15 soft tissue solitary fibrous tumors (SFTs) occurring in patients 24 to 78 years old (average, 50.6 yr). Ten tumors were benign and arose in the head and neck area (three tumors), thigh (two), vulva (two), upper arm (one), lower lag (one), and retroperitoneum (one)
Rhabdomyosarcoma is a malignant soft tissue tumor (sarcoma) originating from striated skeletal muscle. Although rare, it is one of the more common solid cancerous tumors (sarcoma) affecting children. This entity occurs with same frequency in the genitourinary system, head and neck, retroperitoneum/pelvis, and extremity (lower extremity>upper. Diagnosis is made with biopsy showing a spindle cell neoplasm with similar characteristics shared between the osseous and soft tissue forms of this disease. Immunostains are positive for actin and vimentin
Phone: 833-292-BONE (2663) Fax: 973-898-1636 Email: email@example.com Monospecific antibodies and indirect immunofluorescence microscopy were used to investigate the presence of cytoskeletal intermediate filaments of the keratin, vimentin and desmin types in 43 soft‐tissue sarcomas. The results showed that vimentin was present in the neoplastic cells of all types of soft‐tissue sarcomas studied, whereas keratin was absent, the only exception being the. Immunostaining was evaluated as positive when more than 10% of the tumor cell population was positive or, even if the positive cells were less than 10%, the existence 54 Fig. 3. Immunohistochemistry results for vimentin (a-c) and alpha-smooth muscle actin (d-f). a, d Fascicular areas with low cellularity. b, e Myxoid areas. c, f Fascicular.
Leiomyosarcoma is an uncommon soft tissue sarcoma that composed of malignant mesenchymal cells with distinct features of the smooth muscle lineage. Typically affects the uterus and gastrointestinal tract, it can rarely be seen in large blood vessels, lymphatic and glandular duts, the mesentery, the omentum, retroperitoneum, and limbs. Occurrence is particularly rare in the limb region. 10. Hartley AL, Birch JM, Blair V, et al. Patterns of cancer in the families of children with soft tissue sarcoma. Cancer 1993; 72:923-11. Malkin D, Li FP, Strong LC, et al. Germ line p53 mutations in a familial syndrome of breast cancer, sarcomas, and other neoplasms. Science 1990; 250:1233-. 12 RAF family activating fusions have been described as a potentially targetable molecular finding in a subset of soft tissue sarcomas. To further expand upon the landscape of this genetic feature, we describe a novel MTAP-RAF1 activating fusion identified in a S100 positive soft tissue sarcoma. A 51 year old man underwent excision of a soft tissue mass in his foot Soft‐tissue mixed tumor seems to be more closely related to the eccrine type of cutaneous mixed tumor. Therefore, there is a possibility that some cases of soft tissue mixed tumor originate from myoepithelial cells of the deeply located eccrine gland, although there is no positive evidence to support this possibility Liposarcomas are among the most common soft tissue sarcomas, second only to malignant fibrous histiocytoma in incidence, and they account for 16-18% of all soft tissue sarcomas. However, only 3-6% of liposarcomas occur in the head and neck. The incidence of liposarcomas peaks in those aged 30-60 years, and a slight male preponderance is observed